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Catalogue Number
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158395 |
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Antigen/Gene or Protein Targets
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PIK3CA
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Production Details
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Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110αkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
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Conditional
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Yes
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Mouse Genetic Background/Cross History
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Backcrossed onto C57B6/J for more than 10 generations.
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Research Area
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Cancer
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Notes
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Homozygous and heterozygous mice are phenotypically normal.
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