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Pik3ca-H1047R conditional – Flp inducible
RECENTLY UPDATED

Invented by Bart Vanhaesebroeck
Invented at Ludwig Institute for Cancer Research

Info

Catalogue Number 158395
Antigen/Gene or Protein Targets PIK3CA
Production Details Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110αkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
Conditional Yes
Mouse Genetic Background/Cross History Backcrossed onto C57B6/J for more than 10 generations.
Research Area Cancer
Notes Homozygous and heterozygous mice are phenotypically normal.

References

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References: 1 entry

Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.


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References: 1 entry

Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.


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Inventor Information