Transient receptor potential cation channel superfamily M member 2 (TRPM2)-GFP HeLa cell line
Invented by Peter Bradding at University Of Leicester
| Catalogue Number | 158381 |
| Antigen/Gene or Protein Targets | human Transient receptor potential cation channel family member ankyrin 1 (hTRPA1) |
| Parental Line | HeLa |
| Relevance |
The transient receptor potential cation channel family member ankyrin 1 (TRPA1) is an ion channel with high Ca2+ permeability that is activated by numerous noxious stimuli and by multiple products of oxidative stress. TRPA1 is a drug target with antagonists in phase I and II clinical trials. It is considered a potential target in multiple pain conditions including neuropathic, inflammatory and migraine pain, in addition to cough sensitivity, airway inflammation and fibrosis This HeLa cell line is stably transfected with a dual-gene promotor (pCDH-CMV-MCS-EF1-copGFP) to express both GFP and hTRPA1. |
| Conditional | No |
| Research Area | Neurobiology |
| Notes |
Forward TRPA1 Xba1 gggtctagaATGAAGCGCAGCCTGAGGAAG Reverse TRPA1 NotI ggggcggccgcttaAGGCTCAAGATGGTGTGTTTTTGCC |
|
Peter Bradding |
|
Harvinder Virk |