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Catalogue Number
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158349 |
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Antigen/Gene or Protein Targets
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Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20)
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Parental Line
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JU77
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Host
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Human
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Tissue
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Lung
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Disease Keywords
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Lung Cancer
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Model
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Cancer Model
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Production Details
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JU77 cells were incubated with 10ug/ml ADI-PEG20 in RPMI-1640 media supplemented with 2% FBS and re-treated every week. After approximately 2 months, colonies were picked and seeded in a 96-well plate with 10ug/ml ADI-PEG20. A surviving clone (JU777R) was gradually expanded and maintained in 1ug/ml ADI-PEG20.
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Research Area
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Cancer
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Growth/Phenotype Keywords
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Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20).
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Recommended Growing Conditions
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RPMI-1640 media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, and 100ug/ml streptomycin at 37oC/5% CO2.
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Cellosaurus ID
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TBC
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