Anti-Pyruvate Kinase (PKM1 and PKM2) polyclonal antibody
Invented by Abdo Alnabulsi
Invented at Vertebrate Antibodies Limited
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Info
| Catalogue Number | 158063 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Pyruvate Kinase (PKM1 and PKM2) |
| Synonyms | Pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3). |
| Reactivity | FIsh |
| Relevance |
Pyruvate kinase is a key enzyme of the glycolytic pathway, converting phosphoenolpyruvate (PEP) into pyruvate with the generation of one molecule of ATP. Pyruvate kinase is present in four distinct, tissue-specific isozymes in animals: L (liver), R (erythrocytes), M1 (muscle and brain) and M2 (early fetal tissue and most adult tissues). The L and R isozymes are expressed from the gene PKLR, whereas the M1 and M2 isozymes are expressed from the gene PKM2. Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells. The antibody is a useful tool to evaluate the PKM2, a marker of intestinal inflammation. |
| Host | Rabbit |
| Immunogen | Ovalbumin-conjugated synthetic peptide. Peptide immunogen is conserved in all fish species. |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 57 |
| Notes |
Applications and Recommended Usage Conditions: - ELISA recommended to be used at 1/500 dilution. Peptide immunogen as positive control. - WB in progress - IHC recommended to be used at 1/500 dilution. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal. |
| Positive Control | ELISA: Peptide Immunogen WB: in progress IHC: formalin-fixed, paraffin-embedded multi-tissue liver tissues |
| Research Area | Cell Type or Organelle Marker, Immunology |
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Inventor Information
Inventors
|
Abdo Alnabulsi |
