Anti-Orexin polyclonal antibody
Invented by Abdo Alnabulsi
Invented at Vertebrate Antibodies Limited
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Info
| Catalogue Number | 158060 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Orexin |
| Reactivity | FIsh |
| Relevance |
Orexin is a neuropeptide distributed widely among vertebrates. In mammals, orexin and its receptor system are involved in the regulation of food intake, locomotion, and psychomotor activities including the sleep/wakefulness cycle. With regard to non-mammalian vertebrates, there has also been intensive study aimed at the identification and functional characterisation of orexin and its receptor, and recent investigations of the role of orexin have revealed that it exerts behavioral effects in teleost fish. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Fish orexin is a health marker which can be used to monitor fish stock food intake and body weight. |
| Host | Rabbit |
| Immunogen | Ovalbumin-conjugated synthetic peptide. Peptide immunogen is conserved across all fish species. |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 16.6 |
| Notes |
Applications and Recommended Usage Conditions: - ELISA recommended to be used at 1/500 dilution, peptide immunogen - WB recommended to be used at 1/500 dilution, fish serum - IHC recommended to be used at 1/500 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Positive Control | ELISA: peptide immunogen WB: fish serum IHC: formalin-fixed, paraffin-embedded fish liver tissues |
| Research Area | Cell Type or Organelle Marker, Immunology |
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Inventor Information
Inventors
|
Abdo Alnabulsi |
