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Info
| Catalogue Number | 158048 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Cathelicidin 2 |
| Reactivity | Rainbow Trout |
| Relevance |
Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broad-spectrum antimicrobial properties. The antibodies are useful tools to monitor fish CATH2, an antimicrobial peptide associated with infection. |
| Host | Rabbit |
| Immunogen | Polyclonal 1 developed using sequence 1: STTHPLDNCKISPQ (located in the mature peptide) Polyclonal 2 developed using sequence 2: KDSGGPKMGRKNSK (located in the mature peptide) |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 21.9 |
| Notes |
Applications and Recommended Usage Conditions: - ELISA recommended to be used at 1/20000 dilution - WB recommended to be used at 1/500 dilution - IHC recommended to be used at 1/1000 dilution; Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal The antibodies may cross-react with Salmon CATH2. |
| Positive Control | ELISA: Peptide immunogen WB: Fish serum IHC: formalin-fixed, paraffin-embedded gill tissues. FACS: pending |
| Research Area | Cell Type or Organelle Marker, Immunology |
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Inventor Information
Inventors
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Abdo Alnabulsi |
