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DLKP-I cell line

Invented at Dublin City University

Info

Catalogue Number 157985
Parental Line DLKP
Host Human
Tissue Lymph Node
Disease Keywords lung carcinoma
Relevance Sub types of lung carcinoma with well-defined morphology and characteristic studies
Production Details Single cell cloning from DLKP parental population
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords monolayer
Recommended Growing Conditions DMEM/Ham's F12, 5% FCS. Split 1:5, medium change 2-3 times weekly. Freezing method: 10% DMSO in FBS
Positive Control DLKP
Notes Intermediate sub-population cloned at NCTCC from DLKP P5, about 25% of DLKP show DLKP-I morphology. Interconversion between DLKP-M/DLKP-SQ and DLKP-I, tetraploid, fold resistance compared to DLKP: 0.5 Adr; 0.65 Vcr; 0.6 VP-16. Immunocytochemistry: cytokeratin (all) -; GFAP-; vimentin+; neurofilament+; P-gp-; a2-integrin+; desmin-; 703D4+; 704A1+.
Cellosaurus ID CVCL_H738

References

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References: 1 entry

Keenan et al. 2012. Exp Cell Res. 318(5):593-602. PMID: 22281030.


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References: 1 entry

Keenan et al. 2012. Exp Cell Res. 318(5):593-602. PMID: 22281030.


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