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DLKP-pHA MDR 1/A cell line
RECENTLY UPDATED

Invented at Dublin City University

Info

Catalogue Number 157981
Parental Line DLKP
Host Human
Tissue Lymph Node
Disease Keywords lung carcinoma
Relevance heterogeneous lung carcinoma population
Production Details DLKP transfected with a pHaMDR1/A plasmid (full cDNA for human MDR1 gene) and treated with 50mg/ml adriamycin
Conditional Description fold resistance comp. to DLKP parent: 15-fold Adriamycin; 12.2 Vincristin; 13.6 VP-16 (Etoposide).
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords monolayer, slow-growing
Recommended Growing Conditions DMEM/Ham's F12, 5% FCS supplement with 50ng/ml adriamycin at least once/week. Passage 2-3 time weekly, spin after trypsinisation. Freezing: 5% DMSO, freeze at 2x10(6) cells/ml, spin down after reviving
Positive Control DLKP
Notes Epithelial model

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