DLKP-pHA MDR 1/A cell line
Invented at Dublin City University
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Info
| Catalogue Number | 157981 |
| Parental Line | DLKP |
| Host | Human |
| Tissue | Lymph Node |
| Disease Keywords | lung carcinoma |
| Relevance | heterogeneous lung carcinoma population |
| Production Details | DLKP transfected with a pHaMDR1/A plasmid (full cDNA for human MDR1 gene) and treated with 50mg/ml adriamycin |
| Conditional Description | fold resistance comp. to DLKP parent: 15-fold Adriamycin; 12.2 Vincristin; 13.6 VP-16 (Etoposide). |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | monolayer, slow-growing |
| Recommended Growing Conditions | DMEM/Ham's F12, 5% FCS supplement with 50ng/ml adriamycin at least once/week. Passage 2-3 time weekly, spin after trypsinisation. Freezing: 5% DMSO, freeze at 2x10(6) cells/ml, spin down after reviving |
| Positive Control | DLKP |
| Notes | Epithelial model |
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