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DLKP-pHA MDR 1/A
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Info

Catalogue Number 157981
Parental Line DLKP
Host Human
Tissue Lymph Node
Disease Keywords lung carcinoma
Relevance heterogeneous lung carcinoma population
Production Details DLKP transfected with a pHaMDR1/A plasmid (full cDNA for human MDR1 gene) and treated with 50mg/ml adriamycin
Conditional Description fold resistance comp. to DLKP parent: 15-fold Adriamycin; 12.2 Vincristin; 13.6 VP-16 (Etoposide).
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords monolayer, slow-growing
Recommended Growing Conditions DMEM/Ham's F12, 5% FCS supplement with 50ng/ml adriamycin at least once/week. Passage 2-3 time weekly, spin after trypsinisation. Freezing: 5% DMSO, freeze at 2x10(6) cells/ml, spin down after reviving
Positive Control DLKP
Notes Epithelial model

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