Anti-(1-5)-a-L-arabinan [LM6] monoclonal antibody
Invented by Prof Paul Knox from PlantProbes
Invented at University of Leeds
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 157955 |
| Antigen/Gene or Protein Targets | (1-5)-a-L-arabinan |
| Relevance | The antibody will be useful for the localization of arabinans in plant tissue and will have uses in the analyses of pectin structure. |
| Host | Rat |
| Immunogen | A neoglycoprotein: a heptasaccharide of (1→5)-α-l-linked-arabinosyl residues linked to bovine serum albumin. |
| Positive Control | IgG |
| Myeloma Used | IR983F |
| Notes |
Generated using a neoglycoprotein (arabinoheptaose-BSA). Recognises a linear pentasaccharide in (1-5)-α-L-arabinans. It can recognise pectic polysaccharides in several species. It has no cross-reactivity with gum arabic but it may recognize arabinogalactan-proteins (AGPs) in some species. In competitive inhibition ELISAs, antibody binding to (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabino-hexaose. |
| Research Area | Plant Science |
References: 2 entries
Verhertbruggen et al. 2009. Plant J. 59(3):413-25. PMID: 19392693.
Willats et al. 1998. Carbohydr Res. 308(1-2):149-52. PMID: 9675359.
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References: 2 entries
Verhertbruggen et al. 2009. Plant J. 59(3):413-25. PMID: 19392693.
Willats et al. 1998. Carbohydr Res. 308(1-2):149-52. PMID: 9675359.
Add a reference
