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Anti-(1-5)-a-L-arabinan [LM6] monoclonal antibody

Invented by Prof Paul Knox from PlantProbes
Invented at University of Leeds

Info

Catalogue Number 157955
Antigen/Gene or Protein Targets (1-5)-a-L-arabinan
Relevance The antibody will be useful for the localization of arabinans in plant tissue and will have uses in the analyses of pectin structure.
Host Rat
Immunogen A neoglycoprotein: a heptasaccharide of (1→5)-α-l-linked-arabinosyl residues linked to bovine serum albumin.
Positive Control IgG
Myeloma Used IR983F
Notes Generated using a neoglycoprotein (arabinoheptaose-BSA). Recognises a linear pentasaccharide in (1-5)-α-L-arabinans. It can recognise pectic polysaccharides in several species. It has no cross-reactivity with gum arabic but it may recognize arabinogalactan-proteins (AGPs) in some species.

In competitive inhibition ELISAs, antibody binding to (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabino-hexaose.
Research Area Plant Science

References

There are 2 reference entries for this reagent.

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References: 2 entries

Verhertbruggen et al. 2009. Plant J. 59(3):413-25. PMID: 19392693.

Willats et al. 1998. Carbohydr Res. 308(1-2):149-52. PMID: 9675359.


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References: 2 entries

Verhertbruggen et al. 2009. Plant J. 59(3):413-25. PMID: 19392693.

Willats et al. 1998. Carbohydr Res. 308(1-2):149-52. PMID: 9675359.


Add a reference