Anti-(1-5)-a-L-arabinan [LM6-M] monoclonal antibody
Invented by Prof Paul Knox from PlantProbes
Invented at University of Leeds
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Info
| Catalogue Number | 157929 |
| Antigen/Gene or Protein Targets | (1-5)-a-L-arabinan |
| Relevance |
Plant cell walls are highly complex structures composed of load-bearing cellulose microfibrils and interspersed sets of matrix polysaccharides. Pectins, a major component of cell wall matrices, are galacturonic acid-rich polysaccharides and include homogalacturonan and rhamnogalacturonan domains (Caffall and Mohnen, 2009). Pectic rhamnogalacturonan-I (RG-I) is a highly heterogeneous and variable domain of pectin with a rhamnogalacturonan backbone and diverse side chains that are mostly neutral sugars and the major components of which are 1,4-galactosyl and 1,5-arabinosyl residues (Willats et al., 2001; Caffall and Mohnen, 2009). RG-I molecules are known to be structurally highly heterogeneous in cell and developmental contexts and are strongly implicated in influencing the mechanical properties of cell walls and plant materials (Lee et al., 2012; Paniagua et al., 2016; Mikshina et al., 2017) but precise modes of action are not known. This probe, LM6-M, has similar specificity to LM6. However, LM6-M is of a different immunoglobulin isotype and has a higher avidity making it a highly effective mAb for pectic arabinan in in-situ and in-vitro analyses. |
| Host | Rat |
| Immunogen | Sugar beet RG-I oligosaccharides coupled to Bovine Serum Albumin (BSA). |
| Positive Control | IgM |
| Myeloma Used | IR983F |
| Notes | LM6-M was generated using a preparation of sugar beet RG-I oligosaccharides. LM6-M has a similar specificity to LM6 and binds to a linear (1-5)-a-L-arabinan. Unlike LM6 (which is an IgG immunoglobulin) LM6-M is of the IgM isotype. In competitive inhibition ELISA, LM6-M binding to (1-5)-a-L-arabinan was inhibited (50%) by 7.9 µg/ml (1-5)-a-L-arabinopentaose and 3.2 µg/ml (1-5)-a-L-arabinohexaose. LM6-M can recognise pectic polysaccharides in several species. It has no cross-reactivity with gum Arabic but it may recognize arabinogalactan-proteins (AGPs) in some species. |
| Research Area | Plant Science |
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