Anti-UCP2 [A1P2A9*F5] monoclonal antibody
Invented by Abdo Alnabulsi
Invented at Vertebrate Antibodies Limited
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![Anti-UCP2 [A1P2A9*F5] monoclonal antibody hybridoma supernatant used neat to staiin formalin-fixed, paraffin-embedded (FFPE) human kidney tissue. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/fc6d0e78-645d-441d-9ccd-5404e38694c2.png)
![Anti-UCP2 [A1P2A9*F5] monoclonal antibody hybridoma supernatant used neat to stain formalin-fixed, paraffin-embedded (FFPE) human liver tissue. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/95a7b0cd-676e-4adc-9c6e-6e5575919702.jpg)
![Anti-UCP2 [A1P2A9*F5] monoclonal antibody hybridoma supernatant 1:2 dilution used in a western blot of lysate of HEK293 overexpressing UCP2 as a positive control and HEK293-empty vector lysate as negative control. One band at the expected size was detected in the positive control while no band was detected in the negative control lane. Lane 1: 5µl lysate of HEK293 overexpressing UCP2. Lane 2: 5µl empty vector-HEK293 lysate. Lane 3: 8µl MagicMark XP Western Protein Standard.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9b7718ec-685e-4444-8512-fb808174c7f4.png)
![Anti-UCP2 [A1P2A9*F5] monoclonal antibody hybridoma supernatant used neat to staiin formalin-fixed, paraffin-embedded (FFPE) human kidney tissue. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/fc6d0e78-645d-441d-9ccd-5404e38694c2.png)
![Anti-UCP2 [A1P2A9*F5] monoclonal antibody hybridoma supernatant used neat to stain formalin-fixed, paraffin-embedded (FFPE) human liver tissue. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/95a7b0cd-676e-4adc-9c6e-6e5575919702.jpg)
![Anti-UCP2 [A1P2A9*F5] monoclonal antibody hybridoma supernatant 1:2 dilution used in a western blot of lysate of HEK293 overexpressing UCP2 as a positive control and HEK293-empty vector lysate as negative control. One band at the expected size was detected in the positive control while no band was detected in the negative control lane. Lane 1: 5µl lysate of HEK293 overexpressing UCP2. Lane 2: 5µl empty vector-HEK293 lysate. Lane 3: 8µl MagicMark XP Western Protein Standard.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9b7718ec-685e-4444-8512-fb808174c7f4.png)
![Image thumbnail for Anti-UCP2 [A1P2A9*F5] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/fc6d0e78-645d-441d-9ccd-5404e38694c2.png)
![Image thumbnail for Anti-UCP2 [A1P2A9*F5] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/95a7b0cd-676e-4adc-9c6e-6e5575919702.jpg)
![Image thumbnail for Anti-UCP2 [A1P2A9*F5] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9b7718ec-685e-4444-8512-fb808174c7f4.png)
| Catalogue Number | 157894 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2) |
| Reactivity | Human |
| Relevance | A monoclonal antibody against Uncoupling Protein 2 (UCP2). UCPs are members of mitochondrial anion carrier proteins (MACP) family. UCPs create proton leaks across the inner to outer mitochondrial membrane by uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. It is thought to play a role in non-shivering thermogenesis, obesity and diabetes. The main function of UCP2 is the control of mitochondria-derived reactive oxygen species. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide AACTSREAPF |
| Immunogen UniProt ID | P55851 |
| Positive Control | ELISA- Peptide immunogen, Western Blot- HEK293 engineered to overexpress UCP2 cell lysate, Mass spectrometry-peptide analysis of band (western blot), IHC- formalin-fixed, paraffin-embedded multi-tissue microarray (expression was profiled over 54 different human tissues)) |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 36 |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes |
Unpurified hybridoma supernatant has been tested in the following applications: ELISA, neat, peptide-immunogen conjugated to BSA was used as antigen. Western blot, 1:2 dilution, lysate of HEK293 overexpressing UCP2 was used as positive control and HEK293-empty vector lysate was used as negative control. Single band at the expected size was detected in the positive control while no band was detected in the negative control lane. IHC, neat, formalin-fixed paraffin embedded (FFPE) antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. |
| Research Area | Cell Type or Organelle Marker, Cellular Transport, Metabolism |
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Inventor Information
Inventors
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Abdo Alnabulsi |
