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Anti-GW182 [4B6] monoclonal antibody
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Invented by Martin Fritzler at University Of Calgary

Info

Catalogue Number 157878
Applications IF WB
Antigen/Gene or Protein Targets GW182
Synonyms Rinucleotide repeat containing 6A, GW1; GW182; TNRC6; CAGH26
Reactivity Human and Rabbit
Relevance RNA-mediated gene silencing or RNA interference (RNAi) is a key mechanism for controlling mRNA abundance. The RNA-induced silencing complex, or RISC, is a multiprotein complex that incorporates a small interfering RNA (siRNA) or micro RNA (miRNA). Once the RISC associates with its RNA target, it is cleaved by the endonuclease Argonaute 2 (Ago2), resulting in loss of gene function.

GW182 is and RNA-binding protein involved in mRNA metabolism and it is localized to cytoplasmic storage centres known as GW bodies (GWBs). GWBs are also enriched in proteins that are involved in mRNA degradation such as Ago2, and GW182 is thought to contribute and maintain the microenvironment of the RISC.
Host Mouse
Immunogen Recombinant protein
Positive Control IgG1
Molecular Weight (kDa) 182
Notes GW182 is a marker of cytoplasmic GWB foci. Anti-GW182 is used to study the location and function of GW182 protein in tissue culture cells, as well as cryopreserved tissues, and is useful in co-immunoprecipitation studies to identify transient siRNA-protein complexes.
Research Area Cell Type or Organelle Marker, Cell Signaling & Signal Transduction

References

There are 6 reference entries for this reagent.

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References: 6 entries

Gebert et al. 2019. Nat Rev Mol Cell Biol. 20(1):21-37. PMID: 30108335.

Jakymiw et al. 2005. Nat Cell Biol. 7(12):1267-74. PMID: 16284622.

Meister et al. 2005. Curr Biol. 15(23):2149-55. PMID: 16289642.

Sen et al. 2005. Nat Cell Biol. 7(6):633-6. PMID: 15908945.

Eystathioy et al. 2003. Hybrid Hybridomics. 22(2):79-86. PMID: 12831532.

Eystathioy et al. 2002. Mol Biol Cell. 13(4):1338-51. PMID: 11950943.


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References: 6 entries

Gebert et al. 2019. Nat Rev Mol Cell Biol. 20(1):21-37. PMID: 30108335.

Jakymiw et al. 2005. Nat Cell Biol. 7(12):1267-74. PMID: 16284622.

Meister et al. 2005. Curr Biol. 15(23):2149-55. PMID: 16289642.

Sen et al. 2005. Nat Cell Biol. 7(6):633-6. PMID: 15908945.

Eystathioy et al. 2003. Hybrid Hybridomics. 22(2):79-86. PMID: 12831532.

Eystathioy et al. 2002. Mol Biol Cell. 13(4):1338-51. PMID: 11950943.


Add a reference

Inventor Information