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HeLa FRT/TO (YFP-Astrin 4A siRNA res) cell line
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Invented by Duccio Conti, at Queen Mary University of London Viji M. Draviam at Queen Mary University of London

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Catalogue Number 157864
Parental Line HeLa cell line
Synonyms HeLa FRT/TO YFP-Astrin WT cell line conditionally expressing siRNA-resistant YFP-Astrin WT.
Host Human
Tissue Cervix
Model Cancer Model
Production Details HeLa FRT/TO YFP-Astrin 4A cell line conditionally expressing siRNA-resistant YFP-Astrin 4A.

Created by transfection of HeLa Flp recombinase cell line with pCDNA5-FRT/TO-YFP-Astrin 4A expression plasmid, followed by a brief Hygromycin selection and sorting for YFP positive using FACS.
Research Area Cell Type or Organelle Marker, Cell Structure and Motility, Cell Signaling & Signal Transduction, Cell Cycle, Cancer
Notes siRNA oligos to target Astrin mRNA 5’ UTR (GACUUGGUCUGAGACGUGAtt) or Astrin 52 oligo (UCCCGACAACUCACAGAGAAAUU).
Astrin-4A mutant (RVMF to AAAA substitution) was generated using site directed mutagenesis.

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Inventor Information

Inventors

Duccio Conti

Duccio Conti

Viji M. Draviam

Viji M. Draviam

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