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3LL NRAS KO #86 cell line
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Invented by Prof Julian Downward at The Francis Crick Institute

Info

Catalogue Number 157772
Parental Line 3LL aka LL/2 (LLC1) likely origin - please note genetic divergence in relevance information
Host Mouse
Tissue Lung
Model Knock-Out
Relevance Murine Lewis Lung Adenocarcinoma engineered cell line with NRAS knocked out using CRISPR-Cas9. Please note: The authors have established (by DNA sequencing) that these cells are homozygous for the KRAS-G12C & NRAS-Q61H mutations which is not the case for the parent 3LL ATCC line. It is assumed both mutant NRAS and KRAS in 3LL cells drive RAS pathway activation. By knocking out NRAS a tumourigenic cell line with a stronger dependency on KRAS-G12C was generated. Model cell line for study of the effect of KRAS-G12C inhibitors on oncogenic cells.
Research Area Cancer, Cell Cycle, Cell Signaling & Signal Transduction
Growth/Phenotype Keywords Semi-adherent
Recommended Growing Conditions RPMI+10%FCS+1%PS

References

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References: 1 entry

Molina-Arcas et al. 2019. Sci Transl Med. 11(510):. PMID: 31534020.


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References: 1 entry

Molina-Arcas et al. 2019. Sci Transl Med. 11(510):. PMID: 31534020.


Add a reference