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Retinal pigmented epithelial cell line overexpressing HA-FLAG-CXCR4-MYC

Invented by Dr. Allen Liu at University Of Michigan

Info

Catalogue Number 157704
Antigen/Gene or Protein Targets CXCR4
Parental Line Retinal pigmented epithelial cell line
Host Human
Tissue Eye
Relevance Retinal pigment epithelial cells (RPE) stably expressed human CXCR4 when transduced with N-terminal FLAG or N-terminal FLAG and C-terminal MYC tagged CXCR4. RPE cells were used because this cell line has very low CXCR4 expression. Results can be attributed to the overexpression of CXCR4 and not be confounded by endogenous CXCR4. As an experimental model the cells showed overexpressed CXCR4 recapitulate endogenous CXCR4 signalling and internalization dynamics.
Production Details Stable RPE cell lines expressing HA-FLAG-CXCR4 or HA-FLAG-CXCR4-MYC receptors were generated using lentiviral transduction produced from the pLVX vector
Research Area Cell Signaling & Signal Transduction

References

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References: 1 entry

DeNies et al. 2019. Front Cell Dev Biol. 7:77. PMID: 31139626.


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References: 1 entry

DeNies et al. 2019. Front Cell Dev Biol. 7:77. PMID: 31139626.


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Inventor Information