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HEK-ADAM10 CRISPR cell line

Invented by Michael Tomlinson at University Of Birmingham

Info

Catalogue Number 157701
Antigen/Gene or Protein Targets ADAM10
Parental Line HEK 293T
Synonyms A Disintegrin And Metalloproteinase domain-containing protein 10, CRISPR-Cas9
Host Human
Tissue Kidney
Disease Keywords Cancer, Alzheimer’s and inflammatory diseases
Relevance A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines.
Research Area Cancer, Neurobiology, Other
Notes CRISPR edited ADAM 10 HEK-293T cells.

Generation of CRISPR/Cas9-knockout cell line:
A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5’-CACCGATACCTCTCATATTTACAC-3’ and 5’-AAACGTGTAAATATGAGAGGTATC-3’).

References

There are 2 reference entries for this reagent.

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References: 2 entries

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.


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References: 2 entries

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.


Add a reference

Inventor Information