- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||ADAM10|
|Parental Line||HEK 293T|
|Synonyms||A Disintegrin And Metalloproteinase domain-containing protein 10, CRISPR-Cas9|
|Disease Keywords||Cancer, Alzheimer’s and inflammatory diseases|
|Relevance||A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines.|
|Research Area||Cancer, Neurobiology, Other|
CRISPR edited ADAM 10 HEK-293T cells.
Generation of CRISPR/Cas9-knockout cell line:
A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5’-CACCGATACCTCTCATATTTACAC-3’ and 5’-AAACGTGTAAATATGAGAGGTATC-3’).
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