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- Inventor Info
|Antigen/Gene or Protein Targets||Ln12 TCR|
Tumor cells frequently escape from CD8+ T cell recognition by abrogating MHC-I antigen presentation. Deficiency in processing components, like the transporter associated with antigen processing (TAP), results in strongly decreased surface display of peptide/MHC-I complexes. A class of hidden self-antigens known as T cell epitopes associated with impaired peptide processing (TEIPP), which emerge on tumor cells with such processing defects can be investigated with this model.
Using this mouse model, it is possible to investigate the generation of the TEIPP T cell repertoire specifically. These animals harbour rearranged receptors recognizing the Ln12 complex, recognizing a peptide in the context of the non-classical MHC class I molecule Qa-1b (encoded by the H2-T23 gene). The mouse model enables thymus selection and the study of emerging naïve CD8+ T cells.
|Production Details||The Ln12 TCR-transgenic mouse strain was generated by transgenesis of the TCRα and TCRβ genes of the Ln12 T cell clone. The TCRα and TCRβ chains were separately cloned into pCRII-TOPO plasmid vectors using RT-PCR and sequenced. Expression of TCRs was performed by retroviral transduction of the TCR genes in C57BL/6 splenocytes using pMX vector. For transgenesis, the two chains were separately cloned into VA-hCD2 vectors and inserts were subsequently injected in C57BL/6 oocytes. (PMID: 29422902)|
|Breeding Information||These mice breed normally in heterozygous or homozygous background|
|Conditional Description||The expression of the TCR is driven by the CD2 promoter and not conditional|
|Mouse Genetic Background/Cross History||This strain was generated and kept on a C57BL/6 background|
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Thorbald Van Hall