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MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)

Invented by Sarah-Maria Fendt at VIB

Info

Catalogue Number 156524
Antigen/Gene or Protein Targets HRas
Parental Line MCF10A
Synonyms transforming protein p21, h-Ras, H-Ras, HRAS
Tissue Breast
Disease Keywords Cancer
Model Cancer Model
Relevance MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity. This is a control line for the MCF10 H-Ras12 cell line from the same group at the VIB as it has the same pLA expression vector stably expressed but lacking the HRAS gene.
Research Area Cancer
Recommended Growing Conditions DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml−1), 1% streptomycin (50 μg ml−1), 0.5 μg ml−1 hydrocortisone, 100 ng ml−1 cholera toxin, 10 μg ml−1 insulin and 20 ng ml−1 recombinant human EGF.
Notes This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.

References

There are 1 reference entries for this reagent.

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References: 1 entry

Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.


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References: 1 entry

Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.


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Inventor Information

Inventors

Sarah-Maria Fendt

Sarah-Maria Fendt

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