- References (1)
- Inventor Info
|Vector Type||pETSU vector containing an untagged yeast SUMO sequence|
|Antigen/Gene or Protein Targets||A dual tagged clone of the catalytic domain (dtUD1) of the S. cerevisiae SUMO hydrolase. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag.|
The pdtUD1 vector allows the production of recombinant UD1 domain of the S. cerevisiae Ulp1 protein (small ubiquitin related modifier (SUMO) protease). The UD1 domain retains the full SUMO-specific proteolytic activity. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag to allow for easy purification of the recombinant protease.
The dual tags also allow the protease to be removed after cleavage of the SUMO fusion protein generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL, by using subtractive affinity chromatography.
Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein.
Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006
Protein Purification; Protein Biochemistry
There are 1 reference entries for this reagent.View All References
Dr. Patrick Loll