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Immortalized Fibroblastic Reticular cell line

Invented by Dr. Akira Ono at University Of Michigan

Info

Catalogue Number 156505
Parental Line Human lymphatic fibroblasts
Host Human
Tissue Lymph Node
Model Immortalised Line
Relevance Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection.
Production Details Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418.
Research Area Immunology, Virology
Recommended Growing Conditions The cell line was maintained in fibroblast medium containing 2% FBS, P/S, and fibroblast growth supplement and subsequently selected with 1000 µg/ml G418.

References

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References: 1 entry

Murakami et al. 2018. Nat Commun. 9(1):2436. PMID: 29934525.


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References: 1 entry

Murakami et al. 2018. Nat Commun. 9(1):2436. PMID: 29934525.


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Inventor Information