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Catalogue Number
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156505 |
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Parental Line
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Human lymphatic fibroblasts
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Host
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Human
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Tissue
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Lymph Node
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Model
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Immortalised Line
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Relevance
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Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection.
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Production Details
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Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418.
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Research Area
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Immunology, Virology
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Recommended Growing Conditions
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The cell line was maintained in fibroblast medium containing 2% FBS, P/S, and fibroblast growth supplement and subsequently selected with 1000 µg/ml G418.
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