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NHE-3 expressing PS120 cell line

Info

Catalogue Number 156414
Antigen/Gene or Protein Targets Na+/H+ exchanger
Parental Line CCL39
Disease Keywords Diarrhea and constipation
Relevance An isoform of the Na+/H+ exchanger, NHE-3, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:
• NHE-3 is essential in studies related to diarrhea and constipation
Research Area Cell Signaling & Signal Transduction, Drug Discovery & Development

References

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References: 1 entry

Tse et al. 1993. J Biol Chem. 268(16):11917-24. PMID: 7685025.


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References: 1 entry

Tse et al. 1993. J Biol Chem. 268(16):11917-24. PMID: 7685025.


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