Cancer Research Technology
Log in Register
Menu

NHE-1 expressing PS120 cell line

Info

Catalogue Number 156412
Antigen/Gene or Protein Targets Na+/H+ exchanger
Parental Line CCL39
Disease Keywords Cardiac ischemia
Relevance An isoform of the Na+/H+ exchanger, NHE-1, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye.
These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:
• NHE-1 aids in the development of treatment for cardiac ischemia
Research Area Cardiovascular, Drug Discovery & Development, Cell Signaling & Signal Transduction

References

There are 1 reference entries for this reagent.

View All References

References: 1 entry

Tse et al. 1993. J Biol Chem. 268(16):11917-24. PMID: 7685025.


Add a reference

References: 1 entry

Tse et al. 1993. J Biol Chem. 268(16):11917-24. PMID: 7685025.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor