Anti-CD3 [500.A2] monoclonal antibody
Invented at Yale University
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 155246 |
| Applications | N/A |
| Antigen/Gene or Protein Targets | CD3 |
| Reactivity | N/A |
| Relevance |
Monoclonal antibody capable of activating T cells and cloned T cell lines. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. Binding of anti-CD3 (500.A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. |
| Host | Hamster |
| Immunogen | 25-kDa ε chain of the T-cell receptor-associated CD3 complex |
| Immunogen UniProt ID | A0A3Q0CNZ4 |
| Subclass | IgG |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody likely binds CD3 in a location close to a postulated binding site of CD4 to the TCR. Concentration 1mg/ml as standard |
| Research Area | Immunology |
References: 8 entries
Chen et al. 2019. JCI Insight. 4(22):. PMID: 31723059.
Trittel et al. 2019. Sci Rep. 9(1):16362. PMID: 31704965.
Czarnewski et al. 2019. Nat Commun. 10(1):2892. PMID: 31253778.
Zwick et al. 2019. Front Immunol. 10:222. PMID: 30809231.
Shahbaz et al. 2018. PLoS Biol. 16(12):e2006649. PMID: 30550561.
Maj et al. 2017. Nat Immunol. 18(12):1332-1341. PMID: 29083399.
Long et al. 2016. Genes (Basel). 8(1):. PMID: 28035981.
Portoles et al. 1989. J Immunol. 142(12):4169-75. PMID: 2470817.
Add a reference
References: 8 entries
Chen et al. 2019. JCI Insight. 4(22):. PMID: 31723059.
Trittel et al. 2019. Sci Rep. 9(1):16362. PMID: 31704965.
Czarnewski et al. 2019. Nat Commun. 10(1):2892. PMID: 31253778.
Zwick et al. 2019. Front Immunol. 10:222. PMID: 30809231.
Shahbaz et al. 2018. PLoS Biol. 16(12):e2006649. PMID: 30550561.
Maj et al. 2017. Nat Immunol. 18(12):1332-1341. PMID: 29083399.
Long et al. 2016. Genes (Basel). 8(1):. PMID: 28035981.
Portoles et al. 1989. J Immunol. 142(12):4169-75. PMID: 2470817.
Add a reference
