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Anti-CD45 [C363.16A] monoclonal antibody
RECENTLY UPDATED

Info

Catalogue Number 155241
Applications FACS IF IP WB
Antigen/Gene or Protein Targets CD45
Synonyms YCD45R-1
Reactivity Mouse
Relevance
RECENTLY UPDATED
Monoclonal antibody directed against CD45RB isoform of CD45.

Background and Research Application
CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a critical role in TCR and BCR signalling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signalling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis.
Host Rat
Immunogen cloned T cell lines belonging to the helper T cell (Th2) subset, final boost was given with helper/Th2 clone D10
Immunogen UniProt ID
RECENTLY UPDATED
P08575
Subclass IgG
Molecular Weight (kDa) 190 kDa and 220 kDa
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Concentration
1mg/ml as standard
Research Area Immunology

References

There are 7 reference entries for this reagent.

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References: 7 entries
RECENTLY UPDATED

Shao et al. 2020. J Immunol. 204(1):37-48. PMID: 31757864.

Garo et al. 2019. Cell Rep. 28(13):3353-3366.e5. PMID: 31553906.

Timilshina et al. 2019. Cell Rep. 27(10):2948-2961.e7. PMID: 31167140.

Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.

Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.

Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.

Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.


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References: 7 entries
RECENTLY UPDATED

Shao et al. 2020. J Immunol. 204(1):37-48. PMID: 31757864.

Garo et al. 2019. Cell Rep. 28(13):3353-3366.e5. PMID: 31553906.

Timilshina et al. 2019. Cell Rep. 27(10):2948-2961.e7. PMID: 31167140.

Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.

Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.

Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.

Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.


Add a reference

Inventor Information

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