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SIRMu-1 Cell Line
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Catalogue Number 154858
Host Rat
Tissue Eye
Disease Keywords Retinal studies into Müller cell function. Müller cells are a major type of glia cell in the retina of the eye.
Model Immortalised Line
Relevance Both the cellular morphology and overall transcriptome of the SIRMu-1 cells are more similar to primary rat MCs than the commonly used rMC-1 cells
Production Details A monoclonal line dervied from two sequential rounds of single cell cloning by serial dilution.
Research Area Drug Discovery & Development, Neurobiology
Growth/Phenotype Keywords large and flat with a "ghost-like" appearance.
Recommended Growing Conditions MEM*, 20% FBS, 25mM glucose (final concentration), 2mM L-glutamine. *Minimum Essential Medium (+Earle's Salts, -L glutamine, Gibco #11090). Note: The cells also grow fine in 10% FBS and/or 5mM glucose.
Notes No Virus or any pathogen. Not GMO. The cells have not been transformed with any agents. The cells can be passaged 1:3 to 1:5 every 2-4 days. Estimated population doubling times with cultured in 10% and 20% FBS are 36 and 30 hours respectively. The cells can take time to recover after thawing from a frozen stock. After thawing, it is quite normal to have a lot of dead and floating cells. For a vial which contains cells from a third of a confluent T175 flask, it is recommended to thaw into a T25 flask. It can then take up to a week for confluency. Before a complete recovery, the doubling time might be longer than normal.
Cellosaurus ID CVCL_UW38

References

There are 2 reference entries for this reagent.

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References: 2 entries

Kittipassorn et al. 2019. Data Brief. 23:103721. PMID: 31372389.

Kittipassorn et al. 2019. Exp Eye Res. 181:127-135. PMID: 30677389.


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References: 2 entries

Kittipassorn et al. 2019. Data Brief. 23:103721. PMID: 31372389.

Kittipassorn et al. 2019. Exp Eye Res. 181:127-135. PMID: 30677389.


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