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SIRMu-1 Cell Line

Invented by Associate Professor Daniel Peet at The University of Adelaide

Info

Catalogue Number 154858
Host Rat
Tissue Eye
Disease Keywords Retinal studies into Müller cell function. Müller cells are a major type of glia cell in the retina of the eye.
Model Immortalised Line
Relevance Both the cellular morphology and overall transcriptome of the SIRMu-1 cells are more similar to primary rat MCs than the commonly used rMC-1 cells
Production Details A monoclonal line dervied from two sequential rounds of single cell cloning by serial dilution.
Research Area Drug Discovery & Development, Neurobiology
Growth/Phenotype Keywords large and flat with a "ghost-like" appearance.
Recommended Growing Conditions MEM*, 20% FBS, 25mM glucose (final concentration), 2mM L-glutamine. *Minimum Essential Medium (+Earle's Salts, -L glutamine, Gibco #11090). Note: The cells also grow fine in 10% FBS and/or 5mM glucose.
Notes No Virus or any pathogen. Not GMO. The cells have not been transformed with any agents. The cells can be passaged 1:3 to 1:5 every 2-4 days. Estimated population doubling times with cultured in 10% and 20% FBS are 36 and 30 hours respectively. The cells can take time to recover after thawing from a frozen stock. After thawing, it is quite normal to have a lot of dead and floating cells. For a vial which contains cells from a third of a confluent T175 flask, it is recommended to thaw into a T25 flask. It can then take up to a week for confluency. Before a complete recovery, the doubling time might be longer than normal.
Cellosaurus ID CVCL_UW38

References

There are 2 reference entries for this reagent.

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References: 2 entries

Kittipassorn et al. 2019. Data Brief. 23:103721. PMID: 31372389.

Kittipassorn et al. 2019. Exp Eye Res. 181:127-135. PMID: 30677389.


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References: 2 entries

Kittipassorn et al. 2019. Data Brief. 23:103721. PMID: 31372389.

Kittipassorn et al. 2019. Exp Eye Res. 181:127-135. PMID: 30677389.


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Inventor Information

Inventors

Associate Professor Daniel Peet

Associate Professor Daniel Peet

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