SIRMu-1 Cell Line
Invented by Associate Professor Daniel Peet
Invented at The University of Adelaide
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154858 |
| Host | Rat |
| Tissue | Eye |
| Disease Keywords | Retinal studies into Müller cell function. Müller cells are a major type of glia cell in the retina of the eye. |
| Model | Immortalised Line |
| Relevance | Both the cellular morphology and overall transcriptome of the SIRMu-1 cells are more similar to primary rat MCs than the commonly used rMC-1 cells |
| Production Details | A monoclonal line dervied from two sequential rounds of single cell cloning by serial dilution. |
| Research Area | Drug Discovery & Development, Neurobiology |
| Growth/Phenotype Keywords | large and flat with a "ghost-like" appearance. |
| Recommended Growing Conditions | MEM*, 20% FBS, 25mM glucose (final concentration), 2mM L-glutamine. *Minimum Essential Medium (+Earle's Salts, -L glutamine, Gibco #11090). Note: The cells also grow fine in 10% FBS and/or 5mM glucose. |
| Notes | No Virus or any pathogen. Not GMO. The cells have not been transformed with any agents. The cells can be passaged 1:3 to 1:5 every 2-4 days. Estimated population doubling times with cultured in 10% and 20% FBS are 36 and 30 hours respectively. The cells can take time to recover after thawing from a frozen stock. After thawing, it is quite normal to have a lot of dead and floating cells. For a vial which contains cells from a third of a confluent T175 flask, it is recommended to thaw into a T25 flask. It can then take up to a week for confluency. Before a complete recovery, the doubling time might be longer than normal. |
| Cellosaurus ID | CVCL_UW38 |
References: 2 entries
Kittipassorn et al. 2019. Data Brief. 23:103721. PMID: 31372389.
Kittipassorn et al. 2019. Exp Eye Res. 181:127-135. PMID: 30677389.
Add a reference
References: 2 entries
Kittipassorn et al. 2019. Data Brief. 23:103721. PMID: 31372389.
Kittipassorn et al. 2019. Exp Eye Res. 181:127-135. PMID: 30677389.
Add a reference
Inventor Information
Inventors
|
Associate Professor Daniel Peet |
