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Catalogue Number
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154745 |
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Applications
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IHC
WB
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Antigen/Gene or Protein Targets
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Integrin α6B
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Synonyms
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ITGA6; CD49F Antigen
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Reactivity
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Human
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Relevance
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The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Specific loss of this integrin chain in the intestinal epithelium, and thus of their hemidesmosomes, induces long-standing colitis and infiltrating adenocarcinomas.
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Host
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Mouse
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Immunogen
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A mouse was immunized with a synthetic peptide corresponding to a 34 amino acid stretch in the cytoplasmic domain of integrin α6B including an appending N-terminal cysteine: CSRYDDSVPRYHAVRIRKEEREIKDEKYIDNLEKK coupled to keyhole limpet hemocyanin.
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Subclass
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IgG1
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Myeloma Used
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Sp2/0-Ag14
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Recommended Growing Conditions
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RPMI +10% FBS ultra low IgG, 37 ⁰C 5% CO2
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Strain
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Balb/c
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Notes
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Fusion partners: SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse. Recognizes specifically the cytoplasmic domain of integrin subunit α6B. 6B4 stains the basement membrane zone of ducts and acini of salivary gland, kidney tubules epithelium of colonic crypts and weakly stains sweat glands and sebaceous glands.
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Research Area
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Adhesion, Cell Signaling & Signal Transduction
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