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MCF7A estrogen independent breast cancer cell line panel
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Catalogue Number 154646
Antigen/Gene or Protein Targets BCAR3, BCAR4, AKT1
Parental Line MCF7A
Host Human
Tissue Breast
Disease Keywords Mammary adenocarcinoma, estrogen-dependent
Model Cancer Model
Relevance Endocrine therapy of breast cancer has been applied widely and proven to be effective. However, in many instances endocrine treatments ultimately fail due to the development of an estrogen-independent therapy resistant phenotype. To elucidate the molecular mechanism underlying this endocrine therapy failure, the laboratory of Lambert Dorssers applied different genetic screens to identify the main genes conferring estrogen independence.

Out of 15 candidate BCAR genes, several including BCAR3, BCAR4 and AKT1 were shown to directly underlie estrogen independence to MCF7A breast cancer cells. These genes were transfected into the MCF7A breast cancer cell line resulting in a panel of 4 cell lines (Cat No 154636-154638, 154643).

These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
Production Details Full length cDNA of the relevant gene was introduced in the estrogen-dependant MCF7A cell line by transfection with lipofectamine
Conditional No
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS)
Notes The cell lines are also resistant to Geneticin which may be included in the culture to maintain the expression plasmid

References

There are 3 reference entries for this reagent.

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References: 3 entries

Godinho et al. 2011. J Cell Physiol. 226(7):1741-9. PMID: 21506106.

van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.

van Agthoven et al. 1998. EMBO J. 17(10):2799-808. PMID: 9582273.


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References: 3 entries

Godinho et al. 2011. J Cell Physiol. 226(7):1741-9. PMID: 21506106.

van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.

van Agthoven et al. 1998. EMBO J. 17(10):2799-808. PMID: 9582273.


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