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MCF7A AKT1 [MCF7-AKT1] cell line
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Catalogue Number 154636
Antigen/Gene or Protein Targets AKT1
Parental Line MCF7A
Synonyms AKT Serine/Threonine Kinase 1; Protein Kinase B; PKB; RAC
Host Human
Tissue Breast
Disease Keywords Mammary adenocarcinoma, estrogen-dependent
Model Cancer Model
Relevance Breast cancer is widely and effectively treated with endocrine treatment. However, in many disease cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype

Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in MCF7A human breast cancer cells. This cell line is part of a panel of 4 cell lines (Cat No 154636-154638, 154643) which have been transfected with these genes, plus the parental (Cat No 154546)

These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
Production Details Full length AKT1 cDNA was introduced in the estrogen-dependent MCF7A cell line by transfection with lipofectamine
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS)
Notes This cell line is also resistant to Geneticin which may be included in the culture to maintain the expression plasmid

References

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References: 1 entry

van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.


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References: 1 entry

van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.


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