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Monoclonal Antibodies

Anti-Sun1 [BBmSun1 X12.11] monoclonal antibody

Invented by Brian Burke
Invented at A*STAR Accelerate Technologies Pte Ltd
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Image thumbnail for Anti-Sun1 [BBmSun1 X12.11] monoclonal antibody
Image thumbnail for Anti-Sun1 [BBmSun1 X12.11] monoclonal antibody
Image thumbnail for Anti-Sun1 [BBmSun1 X12.11] monoclonal antibody
Image thumbnail for Anti-Sun1 [BBmSun1 X12.11] monoclonal antibody
Image thumbnail for Anti-Sun1 [BBmSun1 X12.11] monoclonal antibody
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Western blot analysis of BBmSun1 X12.11 antibody. Whole cell lysate from mouse (C2C12, immortalized mouse adult fibroblasts (MAFs), SP2/0) cell lines were separated on SDSPAGE gel, transferred to nitrocellulose membrane and blotted with BBmSun1 X12.11. antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight of the longest mouse Sun1 isoform is 102 kDa. The absence of bands in Sun1-null MAFs indicates that the antibody specifically recognizes Sun1. The higher molecular weight band detected by the antibody most likely represent Sun1 dimer while the lower molecular bands might represent alternatively spliced Sun1 variants and/or degradation products. The same blot was stripped and probed for a loading control (beta actin, lower panel). The antibody is not suitable for detecting human Sun1.
Confocal immunofluorescence images of mouse C2C12 cell line fixed with either cold methanol (MeOH) (upper panel) or 4% paraformaldehyde (4% PFA) (lower panel) and stained with BBmSun1 X12.11 antibody. Nuclei were visualized with Hoechst. The BBmSun1 X12.11 does not detect human Sun1.
The antibody specifically detects Sun1. Immortalized wild-type mouse adult fibroblast (MAFs Sun1+/+) (upper panel) and Sun1-null MAFs (Sun1-/-) (lower panel) were fixed in 4% PFA and stained with BBmSun1 X12.11 mouse monoclonal antibody. In Sun1-null MAFs there is a loss of BBSun1 BBmSun1 X12.11 antibody staining signal.
Immunohistochemistry of mouse tissue cryosections. Epididymis (upper two panels) and testis (lower panel) were embedded in OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, incubated in 1% Triton X-100 (in PBS) and stained with anti-Sun1 BBmSun1 X12.11 antibody. Nuclei were visualized with Hoechst dye.
Immunofluorescence analysis of paraffin-embedded mouse tissues. Tissues from adult wild-type (upper two panels) or Sun1-null mice were embedded in paraffin and sectioned. Following heatinduced antigen retrieval (sodium citrate buffer pH 6) the paraffin sections were incubated with 1% Triton X-100 (in PBS) and subsequently stained with mouse monoclonal BBmSun1 X12.11 antibody. Nuclei were visualized with Hoechst.
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Catalogue Number 154471
Applications IHC IF WB
Antigen/Gene or Protein Targets Sun1 type II membrane protein
Synonyms Sun1
Reactivity Mouse
Relevance SUN (Sad1, UNC-84) domain-containing proteins are type II membrane proteins that span the inner nuclear membrane. SUN proteins interact with lamins and other proteins in the nucleoplasm and with KASH (Klarsicht, ANC-1, and Syne homology) domain-containing proteins in the perinuclear space. Since the KASH proteins span the outer nuclear membrane and interact with components of the cytoskeleton, the SUN-KASH complex, also known as the LINC complex (LInker of the Nucleoskeleton and Cytoskeleton), provides a physical link between the nuclear interior and the cytoskeleton. The LINC complex is involved in diverse cellular functions including nuclear architecture, nuclear anchorage and migration, meiotic chromosome movement and cytoskeletal organization.
Not surprisingly, aberrations in the LINC complex have been associated with number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility (1-3).

Sun1 is one of at least five mammalian SUN protein family members and is ubiquitously expressed. Of note, Sun1 undergoes extensive alternative splicing. Human and mouse tissues express simultaneously several isoforms with the presence and prevalence of specific isoforms being tissue dependent (4,5).
Host Mouse
Immunogen Bacterially expressed fragment (close to the Sun domain) of mouse Sun1. The details of the immunogen is proprietary information. The antibody should detect all the known isoforms.
Positive Control C2C12, mouse fibroblast
Molecular Weight (kDa) Mouse 102 kDa; existence of multiple short isoforms
Notes Applications:


- Immunohistochemistry: paraffin sections, cryosections
- Immunocytochemistry/Immunofluoresence: formaldehyde, methanol fixation
- Western Blot

Knock-out validated

Species reactivity: Mouse, does not react with human, however not tested on other species.
Research Area Gene Expression, Genetic Studies Tools

References

There are 5 reference entries for this reagent.

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References: 5 entries

Nishioka et al. 2016. Nucleus. 7(6):572-584. PMID: 27858498.

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Horn et al. 2014. Curr Top Dev Biol. 109:287-321. PMID: 24947240.

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Rothballer et al. 2013. Nucleus. 4(1):29-36. PMID: 23324460.

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Göb et al. 2011. Commun Integr Biol. 4(4):440-2. PMID: 21966565.

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Crisp et al. 2006. J Cell Biol. 172(1):41-53. PMID: 16380439.

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References: 5 entries

Nishioka et al. 2016. Nucleus. 7(6):572-584. PMID: 27858498.

View  

Horn et al. 2014. Curr Top Dev Biol. 109:287-321. PMID: 24947240.

View  

Rothballer et al. 2013. Nucleus. 4(1):29-36. PMID: 23324460.

View  

Göb et al. 2011. Commun Integr Biol. 4(4):440-2. PMID: 21966565.

View  

Crisp et al. 2006. J Cell Biol. 172(1):41-53. PMID: 16380439.

View  

Add a reference

Inventor Information