Cancer Research Technology
Log in Register
Menu

Anti-MBP [BBMBP23.42] monoclonal antibody
NEW

Invented by Brian Burke

Info

Catalogue Number 154470
Applications IF WB
Antigen/Gene or Protein Targets Maltose Binding Protein
Reactivity E.coli
Relevance The E. coli maltose binding protein (MBP) is a product of the malE gene and is part of two multicomponent systems that uptake and sense maltose and higher-order glucose polymers (e.g. maltodextrin). MBP acts in the periplasmic space where it binds with high affinity to maltose/maltodextrin which, in turn binds to (i) a transport apparatus that translocates the MBP ligand across the inner membrane to the cytoplasm and (ii) chemoreceptors causing activation of a signaling pathway that directs taxis toward maltose (1).

MBP is widely used as an affinity tag for purification of recombinant proteins. The reversible and high-affinity binding of MBP to amylose resins enables recovery of recombinant proteins at high purity in a single purification step. The purification and elution are performed under mild conditions, preserving the activity of the recombinant protein. In addition, MBP might increase expression, proper folding, and solubility, of its fusion partner (2–4).
Host Mouse
Immunogen E.coli maltose binding protein (MBP) fusion construct
Immunogen UniProt ID P0AEX9
Positive Control MBP expressing construct in bacteria or mammalian cells
Subclass IgG2a kappa
Molecular Weight (kDa) MBP precursor polypeptide 43 kDa, mature MBP 42 kDa
Notes Immunocytochemistry/Immunofluorescence: formaldehyde, methanol fixation
Western blot: MBP expressed in mammalian and bacteria systems
Research Area Gene Expression, Genetic Studies Tools

References

There are 4 reference entries for this reagent.

View All References

References: 4 entries

Kimple et al. 2013. Curr Protoc Protein Sci. 73:Unit 9.9. PMID: 24510596.

Riggs et al. 2001. Curr Protoc Mol Biol. Chapter 16:Unit16.6. PMID: 18265133.

Kapust et al. 1999. Protein Sci. 8(8):1668-74. PMID: 10452611.

Boos et al. 1998. Microbiol Mol Biol Rev. 62(1):204-29. PMID: 9529892.


Add a reference

References: 4 entries

Kimple et al. 2013. Curr Protoc Protein Sci. 73:Unit 9.9. PMID: 24510596.

Riggs et al. 2001. Curr Protoc Mol Biol. Chapter 16:Unit16.6. PMID: 18265133.

Kapust et al. 1999. Protein Sci. 8(8):1668-74. PMID: 10452611.

Boos et al. 1998. Microbiol Mol Biol Rev. 62(1):204-29. PMID: 9529892.


Add a reference

Inventor Information