16E6/E7-HSK cell line
Invented by Prof Aloysius Klingelhutz from The University Of Iowa
Invented at The University Of Iowa
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154462 |
| Antigen/Gene or Protein Targets | Used as an immortal adult human skin keratinocyte line for pathogenesis and inflammation studies |
| Parental Line | Keratinocytes isolated from punch biopsies of the skin from the buttocks |
| Host | Human |
| Tissue | Skin |
| Disease Keywords | Cancer |
| Relevance | Expresses skin cell markers; proinflammatory. Tissue is adult skin cells. |
| Production Details | Cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418 |
| Conditional | No |
| Research Area | Cancer, Microbiology, Virology |
| Growth/Phenotype Keywords | Upregulation of telomerase and greatly extended lifespan (greater than 5 times the lifespan of untransduced keratinocytes) with no apparent crisis or slow down in growth. Despite upregulation of telomerase, E6/E7 transduced keratinocytes exhibited significant telomere shortening with passaging in culture |
| Recommended Growing Conditions | G418 selection. |
References: 2 entries
Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498.
Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA compon
Europe PMC ID: 19558498
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References: 2 entries
Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498.
Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA compon
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