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MDA-MB-231 TetOn-3G ROCK1 cell line
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Invented by Heather Mckinnon, Justin Bower, Daniel Croft

Info

Catalogue Number 154150
Antigen/Gene or Protein Targets ROCK1
Parental Line MDA-MB-231 D3H2LN-Luc cell line
Synonyms Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I, p160 ROCK-1, p160ROCK
Host Human
Tissue Breast
Disease Keywords Triple negative breast cancer (TNBC)
Model Transgenic
Relevance This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK1. ROCK1 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK1 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion.

The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Useful for the study of breast cancer cell cytoskeleton reorganisation and cell migration. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
Conditional Yes
Conditional Description MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK1. Therefore ROCK1 expression is induced in the presence of doxycycline.
Research Area Cancer, Cell Structure and Motility, Drug Discovery & Development, Genetic Studies Tools
Growth/Phenotype Keywords Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
Recommended Growing Conditions 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine

References

There are 2 reference entries for this reagent.

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References: 2 entries

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.

A novel small-molecule MRCK inhibitor blocks cancer cell invasion.

Europe PMC ID: 25288205


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References: 2 entries

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.

A novel small-molecule MRCK inhibitor blocks cancer cell invasion.


Add a reference

Inventor Information

Inventors

Heather Mckinnon

Heather Mckinnon

Justin Bower

Justin Bower

Daniel Croft

Daniel Croft

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