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MDA-MB-231 D3H2LN-Luc TetOn-3G cell line
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Invented by Heather Mckinnon, Justin Bower, Daniel Croft

Info

Catalogue Number 154147
Parental Line MDA-MB-231 D3H2LN-Luc
Host Human
Tissue Breast
Disease Keywords Triple negative breast cancer (TNBC)
Model Reporter
Relevance This cell line contains a tetracyline inducible promoter construct ready for cloning in a gene of interest. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Unbekandt et al., 2014 introduced their genes of interest, the kinases MRCK╬▓, ROCK1 and ROCK2 under the tetracycline inducible promoter, to study the impact of their expression and inhibition on migration of the cells.

Useful for the study of breast cancer cell cytoskeleton reorganisation, cell migration and the development of therapies that inhibit the ability of primary tumours to metastasize. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
Production Details Generated an MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G
Conditional Yes
Conditional Description When a gene of interest is introduced under the control of the tetracycline (Tet)-regulated transactivator Tet-On 3G expression can be induced by administration of doxycycline. Luciferase expression is non-conditional.
Research Area Cancer, Cell Structure and Motility, Drug Discovery & Development, Genetic Studies Tools
Growth/Phenotype Keywords Adherent cell line
Recommended Growing Conditions 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
Positive Control HeLa TetOn-3G

References

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References: 1 entry

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.


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References: 1 entry

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.


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Inventor Information

Inventors

Heather Mckinnon

Heather Mckinnon

Justin Bower

Justin Bower

Daniel Croft

Daniel Croft

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