- References (2)
- Inventor Info
|Applications||ELISA IF IP WB|
|Antigen/Gene or Protein Targets||Nucleocapsid Protein of MERS-CoV|
|Synonyms||Anti-Nucleocapsid Protein of MERS CoV [7H6]|
The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus.
One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs).
However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses.
The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses.
Recommended to aliquot and store antibodies at -20C for prolonged usage
|Research Area||Apoptosis and Programmed Cell Death, Cell Cycle, Microbiology, Other|
There are 2 reference entries for this reagent.View All References
Yee Joo Tan