Anti Nucleocapsid Protein of MERS CoV [7H6] Monoclonal Antibody
Invented by Yee Joo Tan
Invented at A*STAR Accelerate Technologies Pte Ltd
- References (2)
- Inventor Info
|Applications||ELISA IF IP WB|
|Antigen/Gene or Protein Targets||Nucleocapsid Protein of MERS-CoV|
|Synonyms||Anti-Nucleocapsid Protein of MERS CoV [7H6]|
Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus.
Background and Research Application
The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus.
One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs).
However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses.
The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses.
|Immunogen UniProt ID||R9UM87|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
Recommended to aliquot and store antibodies at -20C for prolonged usage
1mg/ml as standard
|Research Area||Apoptosis and Programmed Cell Death, Cell Cycle, Microbiology, Other|
There are 2 reference entries for this reagent.View All References
Yee Joo Tan