Anti Nucleocapsid Protein of MERS CoV [7H6] Monoclonal Antibody
Invented by Yee Joo Tan
Invented at A*STAR Accelerate Technologies Pte Ltd
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154123 |
| Applications | ELISA IF IP WB |
| Antigen/Gene or Protein Targets | Nucleocapsid Protein of MERS-CoV |
| Synonyms | Anti-Nucleocapsid Protein of MERS CoV [7H6] |
| Reactivity | Mouse |
| Relevance |
Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus. Background and Research Application The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus. One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs). However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses. The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses. |
| Host | Mouse |
| Immunogen | GST-N196-413 protein |
| Immunogen UniProt ID | R9UM87 |
| Subclass | IgG2b |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Recommended to aliquot and store antibodies at -20C for prolonged usage Concentration 1mg/ml as standard |
| Research Area | Apoptosis and Programmed Cell Death, Cell Cycle, Microbiology, Other |
References: 2 entries
Aboagye et al. 2018. Biosci Rep. 38(5):. PMID: 30242057.
Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.
Europe PMC ID: 30242057
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References: 2 entries
Aboagye et al. 2018. Biosci Rep. 38(5):. PMID: 30242057.
Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.
Add a reference
Inventor Information
Inventors
|
Yee Joo Tan |
