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Immortalised Human Keratinocyte Cell Line

Invented by Professor. Pranab K

Info

Catalogue Number 154113
Parental Line Human foreskin
Host Human
Tissue Kidney
Model Immortalised Line
Relevance Immortalised human keratinocytes are widely used for studies of skin biology and differentiation
Production Details Keratinocyte cultures were establised from neonatal foreskin using standard methods. Keratinocytes were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
Research Area Cell Type or Organelle Marker
Growth/Phenotype Keywords Cells were routinely passaged 1:4 at confluency
Recommended Growing Conditions Dulbecco’s Modified Eagle Medium [High Glucose, 2mM L-glutamine] supplemented with 10% Fetal Calf Serum

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Inventor Information

Inventors

Professor. Pranab K

Professor. Pranab K

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