Immortalised Human Keratinocyte Cell Line
Invented by Professor. Pranab K
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Info
| Catalogue Number | 154113 |
| Parental Line | Human foreskin |
| Host | Human |
| Tissue | Kidney |
| Model | Immortalised Line |
| Relevance | Immortalised human keratinocytes are widely used for studies of skin biology and differentiation |
| Production Details | Keratinocyte cultures were establised from neonatal foreskin using standard methods. Keratinocytes were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml. |
| Research Area | Cell Type or Organelle Marker |
| Growth/Phenotype Keywords | Cells were routinely passaged 1:4 at confluency |
| Recommended Growing Conditions | Dulbecco’s Modified Eagle Medium [High Glucose, 2mM L-glutamine] supplemented with 10% Fetal Calf Serum |
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