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Immortalised Human Fibroblast Cell Line

Invented by Professor. Pranab K

Info

Catalogue Number 154112
Parental Line Human foreskin
Host Human
Tissue Fibroblast
Model Immortalised Line
Relevance Immortalised human fibroblasts are widely employed as a model system for cell and molecular biology studies
Production Details Fibroblast cultures were establised from neonatal foreskin using standard methods. Fibroblasts were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
Research Area Cell Type or Organelle Marker
Growth/Phenotype Keywords Cells were routinely passaged 1:4 at confluency
Recommended Growing Conditions DMEM:M199 (1:4) supplemented with 10% FBS

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Professor. Pranab K

Professor. Pranab K

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