- References (3)
- Inventor Info
|Parental Line||Non-lesional skin of vitiligo patient|
|Relevance||Vitiligo is a long-term skin condition characterised by patches of the skin losing their pigment. The patches of skin affected become white and usually have sharp margins. The exact cause of vitiligo is unknown however it is believed to be due to genetic susceptibility that is triggered by an environmental factor such that an autoimmune disease occurs. This results in the destruction of skin pigment cells. Risk factors include a family history of the condition or other autoimmune diseases, such as hyperthyroidism, alopecia areata, and pernicious anemia. Globally about 1% of people are affected by vitiligo. Primary melanocytes isolated from the non-lesional skin of a patient with vitiligo can be used as a tool for research in combination with those isolated from the peri-lesional skin (Cat No:154107).|
|Production Details||Autologous skin was taken from patients with vitiligo using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).|
|Research Area||Cell Type or Organelle Marker, Immunology|
|Recommended Growing Conditions||HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)|
There are 3 reference entries for this reagent.View All References
References: 3 entries
Wańkowicz-Kalińska et al. 2003. Lab Invest. 83(5):683-95. PMID: 12746478.
Immunopolarization of CD4+ and CD8+ T cells to Type-1-like is associated with melanocyte loss in human vitiligo.
Europe PMC ID: 12746478
Verhagen et al. 1997. J Immunol. 159(9):4474-83. PMID: 9379047.
Add a reference
References: 3 entries
Professor Pranab K Das