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Halo nevus Melanocyte Cell Line

Invented by Professor Pranab K Das at

Info

Catalogue Number 154105
Parental Line Halo nevus
Synonyms Sutton nevi, Leukoderma acquisitum centrifugum, Perinevoid vitiligo
Host Human
Disease Keywords Halo Nevus
Model Primary line
Relevance Halo nevus is a mole that is surrounded by a depigmented ring or 'halo'. The formation of a halo surrounding a nevi us believed to occur when CD8+ T lymphocytes destroy the pigment producing melanocytes cells however the cause for the attack is unknown. Halo nevi are estimated to be present in approximately 1% of the general population, and are found to be more prevalent in people with vitiligo, malignant melanoma, or Turner syndrome. Primary melanocytes isolated from a halo nevus can be used as a tool for research.
Production Details Autologous skin was taken from halo nevus using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Conditional No
Research Area Cell Type or Organelle Marker, Immunology
Recommended Growing Conditions HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)

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Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

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Invented at