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Halo nevus Melanocyte Cell Line

Invented by Professor Pranab K Das

Info

Catalogue Number 154105
Parental Line Halo nevus
Synonyms Sutton nevi, Leukoderma acquisitum centrifugum, Perinevoid vitiligo
Host Human
Disease Keywords Halo Nevus
Model Primary line
Relevance Halo nevus is a mole that is surrounded by a depigmented ring or 'halo'. The formation of a halo surrounding a nevi us believed to occur when CD8+ T lymphocytes destroy the pigment producing melanocytes cells however the cause for the attack is unknown. Halo nevi are estimated to be present in approximately 1% of the general population, and are found to be more prevalent in people with vitiligo, malignant melanoma, or Turner syndrome. Primary melanocytes isolated from a halo nevus can be used as a tool for research.
Production Details Autologous skin was taken from halo nevus using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Conditional No
Research Area Cell Type or Organelle Marker, Immunology
Recommended Growing Conditions HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)

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Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

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