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Piebaldism Melanocyte Cell Line

Invented by Professor Pranab K Das

Info

Catalogue Number 154104
Parental Line Lesional skin of piebaldism patient
Host Human
Disease Keywords Piebaldism
Model Primary line
Relevance Piebaldism is a rare autosomal dominant disorder of melanocyte development. Common characteristics include a congenital white forelock, scattered normal pigmented and hypo pigmented macules and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
Production Details Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Conditional No
Research Area Cell Type or Organelle Marker, Immunology
Recommended Growing Conditions HAMs F10 supplemented with 10µg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)

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Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

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