Cancer Research Technology
Log in Register
Menu

Immortalised AID deficient B Cell Line

Invented by Professor Pranab K Das

Info

Catalogue Number 154103
Parental Line B cells from AID deficient patient
Synonyms Activation-induced Cytidine Deaminase, AICDA
Host Human
Disease Keywords Hyper IgM Syndrome type 2
Model Immortalised Line
Relevance Hyper IgM syndromes is a group of primary immune deficiency disorders characterised by defective CD40 signalling; via B cells affecting class switch recombination (CSR) and somatic hyper mutation. Immunoglobulin (Ig) class switch recombination deficiencies are characterised by elevated serum Immunoglobulin M (IgM) levels and a considerable deficiency in Immunoglobulins G (IgG), A (IgA) and E (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and IgA and normal or elevated IgM, leading to increased susceptibility to infections. Hyper IgM Syndrome type 2 (autosomal recessive), is characterised by mutations of the AICDA gene. In this type, B cells cannot recombine genetic material to change heavy chain production
Production Details B Cells were isolated from PBMCs of AID deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.
Conditional No
Research Area Cell Type or Organelle Marker, Immunology
Growth/Phenotype Keywords Maintain at 3x105 to 7x105 cells per ml for optimal growth. Replenish growth medium twice per week
Recommended Growing Conditions RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50µg/ml streptomycin
Notes Can be used as antigen presenting cells

References

There are 0 reference entries for this reagent.

References: 0 entry

There is no reference for this reagent yet, feel free to use the button below to suggest one.

Add a reference

References: 0 entry

There is no reference for this reagent yet, feel free to use the button below to suggest one.

Add a reference

Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

Add an inventor

Invented at