- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets
|Diffuse Large B-Cell Lymphoma
|Zinc Finger Protein 51, B Cell CLL/Lymphoma 6, Protein LAZ-3
|Diffuse Large B-Cell Lymphoma (DLBCL) is a B-Cell non-Hodgkins Lymphoma, the fifth most common cancer in the western world. DLBCL is a poorly understood and aggressive disease with ongoing research for the development of effective therapies. This mouse model mimics a chromosomal translocation found in approximately 50% of human DLBCL cases.
|The IμHABCL6 targeting vector was constructed by subcloning a HA-tagged murine BCL6 cassette into the pPNT vector, downstream of the IgH Iμ promoter (1.1Kb PCR fragment) and 5′ to a loxP-flanked stop cassette containing a neomycin-resistance gene (neoR). A ∼10 Kb EcoRI fragment including the four Cμ exons was then isolated from pEco1.1Cμ vector and subcloned downstream to the neoR cassette. The targeting vector was electroporated in the ES cell line 129/Sv, and Neo-resistant, homologous recombinant clones were identified. After Cre mediated excision of the neoR cassette in vitro by transient transfection of a Cre-expressing plasmid, homologous recombinant ES cell clones were injected into blastocysts from C57BL/6 mice. Chimeric mice obtained from ES clones transmitted the knockin allele through the germline and were all backcrossed onto a C57BL/6 background (1–8 generations)
|Mouse Genetic Background/Cross History
|Knockin mouse expressing BCL6 constitutively in B cells under control of the immunoglobulin Iµ promotor
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