MDR KO Mouse
Invented at The Trustees of Columbia University in the City of New York
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154094 |
| Antigen/Gene or Protein Targets | Minimal deleted region of h13q14/m14qC3 |
| Disease Keywords | Chronic Lymphoblastic Leukemia |
| Model | Knock-Out |
| Relevance | Deletion of human chromosomal region 13q14 (mouse 14qC3) represents the most common genetic aberration in B-cell chronic lymphocytic leukaemia (CLL), a neoplasm of mature B lymphocytes. 13q14 deletions are commonly large and heterogeneous in size and affect multiple genes. Contained within the 13q14 region is the 0.11 megabase-long MDR, which encompasses the DLEU2 gene and miR-15a/16-1 cluster. Deletion of the MDR in this model organism recapitulates the full spectrum of CLL-associated lymphoproliferations in humans. |
| Production Details | A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele |
| Conditional | No |
| Strain | C57BL/6 |
| Mouse Genetic Background/Cross History | 129/Sv-C57Bl/6 mixed |
| Zygosity | Homozygous |
| Research Area | Cancer, Immunology |
| Notes | Knockout mouse of the MDR chromosomal region in m14qC3/h13q14, which encodes the DLEU2 gene and the miR15a/16-1 microRNA cluster |
References: 2 entries
Klein et al. 2010. Cancer Cell. 17(1):28-40. PMID: 20060366.
The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.
Europe PMC ID: 20060366
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References: 2 entries
Klein et al. 2010. Cancer Cell. 17(1):28-40. PMID: 20060366.
The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.
Add a reference
