Anti-Mycobacterial 30-kDa [A2h11]
Invented by Professor Pranab K Das
Invented at
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 154072 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Mycobacterial 30-kDa |
| Reactivity | Mycobacterium bovis and Mycobacterium avium |
| Relevance | Mycobacterial 65-kDa hsp and the actively secreted antigen 85 complex of 30 -kDa region proteins are considered to be major immune targets in mycobacterial diseases. This antibody recognises hsp65 and antigens 85A (MPT44) and 85B (MPT59). This antibody stains clear rod-shaped M.leprae bacilli within macrophages. |
| Host | Mouse |
| Immunogen | 30-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114) |
| Positive Control | M.tuberculosis |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | RPMI 1640 |
| Strain | Balb/c |
| Notes | Reacts with M.bovis BCG, M.tuberculosis complex, M.avium complex and M.leprae. Does not react with 65 kDa protein from E.coli. Epitope on mycobacterial 65 kDa heat shock protein mapped to residues in the carboxy-terminal region of amino acid residues 303-424. Epitope of the secreted antigen 85 complex mapped to amino acid residues 55-266. |
| Research Area | Bacteriology, Immunology, Microbiology |
References: 6 entries
Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.
Europe PMC ID: 7682995
Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.
Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.
Europe PMC ID: 1280626
Rambukkana et al. 1992. Infect Immun. 60(11):4517-27. PMID: 1383151.
Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae.
Europe PMC ID: 1383151
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References: 6 entries
Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.
Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.
Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.
Rambukkana et al. 1992. Infect Immun. 60(11):4517-27. PMID: 1383151.
Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae.
Add a reference
