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Anti-Mycobacterial 30-kDa [A2h11]

Invented by Professor Pranab K Das

Info

Catalogue Number 154072
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Mycobacterial 30-kDa
Reactivity Mycobacterium bovis and Mycobacterium avium
Relevance Mycobacterial 65-kDa hsp and the actively secreted antigen 85 complex of 30 -kDa region proteins are considered to be major immune targets in mycobacterial diseases. This antibody recognises hsp65 and antigens 85A (MPT44) and 85B (MPT59). This antibody stains clear rod-shaped M.leprae bacilli within macrophages.
Host Mouse
Immunogen 30-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114)
Positive Control M.tuberculosis
Subclass IgG1
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640
Strain Balb/c
Notes Reacts with M.bovis BCG, M.tuberculosis complex, M.avium complex and M.leprae. Does not react with 65 kDa protein from E.coli. Epitope on mycobacterial 65 kDa heat shock protein mapped to residues in the carboxy-terminal region of amino acid residues 303-424. Epitope of the secreted antigen 85 complex mapped to amino acid residues 55-266.
Research Area Bacteriology, Immunology, Microbiology

References

There are 6 reference entries for this reagent.

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References: 6 entries

Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.

The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.

Europe PMC ID: 7682995

Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.

Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.

Europe PMC ID: 1280626

Rambukkana et al. 1992. Infect Immun. 60(11):4517-27. PMID: 1383151.

Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae.

Europe PMC ID: 1383151


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References: 6 entries

Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.

The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.

Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.

Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.

Rambukkana et al. 1992. Infect Immun. 60(11):4517-27. PMID: 1383151.

Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae.


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Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

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