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Monoclonal Antibodies

Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody

Invented by Dr Bernhard B. Singer from LeukoCom
Invented at LeukoCom
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Image thumbnail for Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody
Image thumbnail for Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody
Image thumbnail for Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody
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Flow cytometry histogram: CHO-CEACAM1 cells were stained with purified human rec. antibody anti CEACAM1 followed by mAb 42-1 (thick line) or isotype control mAb (thin line), followed by Streptavidin-FITC. After washing samples were analyzed by flow cytometer. Dead cells were discriminated by staining with propidium iodide (PI) and excluded from the analysis.
Western Blot: Two different batches of FCS were separated under non-reducing condition in a 7% Tricine gel, blotted to a nitrocellulose membrane and after blocking with 1% milk/PBS incubated with 3 µg/ml mAb42-1 diluted in blocking buffer over night at 4°C. Subsequently, membrane was washed twice, incubated with HRP coupled goat anti mouse antibody, washed and visualized by ECL detection. In both samples bovine IgG was detected at a size of approximately 150 kDa.
Indirect Enzyme-Linked Immunosorbent Assay (ELISA): Indicated antigens were coated onto wells. After blocking with 1% BSA/PBS biotin coupled anti bovIgG mAb42-1 (5 µg/ml) was added. After washing HRP-coupled streptavidin was incubated, washed and visualized by TMB detection.
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Image thumbnail for Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody Image thumbnail for Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody Image thumbnail for Anti- Bovine/Ovine/Goat/Human IgG [42.1] monoclonal antibody
Catalogue Number 154004
Applications ELISA FACS WB
Antigen/Gene or Protein Targets Bovine, ovine, goat, human IgG
Reactivity Bovine, Human, Goat, Mouse, Rat, Rabbit and Sheep
Host Mouse
Immunogen Purified bovine IgG from FCS
Subclass IgG1 kappa
Myeloma Used NS1/0
Notes Protein G purified mouse monoclonal antibody.

Potential use as a secondary antibody for broad spectra of primary antibodies. If were coupled to a fluorochrome or biotin, could be used for detecting primary antibodies in flow cytometry, Western Blots, ELISA, IHC etc.
Research Area Cancer, Immunology

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