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Monoclonal Antibodies

Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody

Invented by Dr Bernhard B. Singer from LeukoCom
Invented at LeukoCom
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Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody
Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody
Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody
Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody
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Flow cytometry histogram: CHO-CEACAM1 transfectants were stained with purified mAb P2-4H (5 µg/ml; thick line) or isotype matched control mAb (thin line), followed by goat anti-mouse-FITC and analyzed by flow cytometer. Dead cells were discriminated by staining with propidium iodide (PI) and excluded from the analysis.
Flow Cytometry: Indicated CEACAM transfectants were stained with purified mAb P2-4H (5 µg/ml) followed by goat anti-mouse-FITC and analyzed by flow cytometer. Dead cells were discriminated by staining with propidium iodide (PI) and excluded from the analysis.
Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Indicated antigens were coated onto wells. After blocking with 2% BSA/PBS mAb P2-4H (5 µg/ml) was incubated with the antigens. After washing HRP-coupled goat anti mouse antibody was incubated and visualized by TMB detection.
CHO wt and CHO-CEACAM1 transfectant were separated under reducing condition in a 7% Tricine gel, blotted to a nitrocellulose membrane and after blocking with 1% milk/PBS incubated with 3 µg/ml mAb P2-4H diluted in blocking buffer over night at 4°C. Subsequently, membrane was washed twice, incubated with HRP coupled goat anti rabbit antibody, washed and visualized by ECL detection.
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Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody Image thumbnail for Anti-CEACAM1/3/5/6 (CD66a/d/e/c) [D2-4H] monoclonal antibody
Catalogue Number 154000
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets Human CEACAM 1, 3, 4, 6
Reactivity Human
Host Mouse
Immunogen Human CEA derivative
Subclass IgG1 kappa
Myeloma Used NS1/0
Notes CEA-related cell adhesion molecules (CEACAMs) are members of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. All CEACAMs are heavily glycosylated. The CEACAM subgroup in humans consists of 12 members composed of a single immunoglobulin variable (IgV)-like N-terminal (N) domain followed by zero to six Ig constant (IgC)-like domains of A and B subtypes and one member which consists of two IgC-like domains and two IgV-like domains, one at each end of the molecule.

CEACAMs show a very heterogeneous expression pattern and can be expressed in several leukocyte-subtypes, most epithelia and endothelia. As their expression pattern, CEACAM mediated functions are very heterogeneous and range from cell-cell communication, proliferation, apoptosis to differentiation and contact-inhibition. Most CEACAMs also serve as pathogen receptors.
Research Area Cancer, Immunology

References

There are 5 reference entries for this reagent.

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References: 5 entries

1.) Singer B.B.: CEACAMs. Encyclopedia of Signaling Molecules. 2016.

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Javaheri et al. 2016. Nat Microbiol. 2:16189. PMID: 27748768.

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Helicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs.

Europe PMC ID: 27748768

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Beauchemin et al. 2013. Cancer Metastasis Rev. 32(3-4):643-71. PMID: 23903773.

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Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in cancer progression and metastasis.

Europe PMC ID: 23903773

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References: 5 entries

1.) Singer B.B.: CEACAMs. Encyclopedia of Signaling Molecules. 2016.

View  

Javaheri et al. 2016. Nat Microbiol. 2:16189. PMID: 27748768.

View  

Helicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs.

View  

Beauchemin et al. 2013. Cancer Metastasis Rev. 32(3-4):643-71. PMID: 23903773.

View  

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in cancer progression and metastasis.

View  

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Inventor Information