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- References (2)
- Inventor Info
Info
| Catalogue Number | 153944 |
| Antigen/Gene or Protein Targets | Escherichia coli cells can metabolize glucose but can accumulate lactic acid from xylose. |
| Classification | Escherichia coli |
| Bacterial Resistance | Chloramphenicol |
| Relevance | Microbial fermentation processes are used to generate a myriad of comedic chemicals representing market segments valued in the billions of dollars. Microbial metabolic pathways are not though, naturally optimal for the generation of a desired chemical, rather they have evolved for the benefit of the organism. Metabolic engineering is the targeted and rational alteration of metabolism, and it involves the redirection of intra-cellular processes to generate a new product or a product in much higher yields and/or faster rates. UGA researchers have developed a collection of engineered E. coli strains that are capable of (i) producing pyruvate, (ii) used in the co-fermentation – at the same rate - of mixed pentoses and hexoses, or (iii) reducing the formation of acetate by-product of fermentations, under different metabolic conditions. These strains are optimal starting points for the development of novel derivative strains for industrial use |
| Genomic Feature | MG1655 ΔptsG763::(FRT) ΔmanZ743::(FRT) Δglk-726::(FRT) ΔpflB::Cam |
| Notes | Lysogeny broth: 10g/L tryptone, 10g/L NaCl, 5g/L yeast extract, pH adjusted to 7.0, aerobic 37C. Provided as lyophilized culture. |
| Research Area | Metabolism, Microbiology, Bacteriology |
References: 2 entries
Eiteman et al. 2009. Biotechnol Bioeng. 102(3):822-7. PMID: 18828178.
A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose.
Europe PMC ID: 18828178
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References: 2 entries
Eiteman et al. 2009. Biotechnol Bioeng. 102(3):822-7. PMID: 18828178.
A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose.
Add a reference
Inventor Information
Inventors
|
Mark Eiteman |
