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E. coli strain ALS1073

Invented by Mark Eiteman

Info

Catalogue Number 153944
Antigen/Gene or Protein Targets Escherichia coli cells can metabolize glucose but can accumulate lactic acid from xylose.
Classification Escherichia coli
Bacterial Resistance Chloramphenicol
Relevance Microbial fermentation processes are used to generate a myriad of comedic chemicals representing market segments valued in the billions of dollars. Microbial metabolic pathways are not though, naturally optimal for the generation of a desired chemical, rather they have evolved for the benefit of the organism. Metabolic engineering is the targeted and rational alteration of metabolism, and it involves the redirection of intra-cellular processes to generate a new product or a product in much higher yields and/or faster rates. UGA researchers have developed a collection of engineered E. coli strains that are capable of (i) producing pyruvate, (ii) used in the co-fermentation – at the same rate - of mixed pentoses and hexoses, or (iii) reducing the formation of acetate by-product of fermentations, under different metabolic conditions. These strains are optimal starting points for the development of novel derivative strains for industrial use
Genomic Feature MG1655 ΔptsG763::(FRT) ΔmanZ743::(FRT) Δglk-726::(FRT) ΔpflB::Cam
Notes Lysogeny broth: 10g/L tryptone, 10g/L NaCl, 5g/L yeast extract, pH adjusted to 7.0, aerobic 37C. Provided as lyophilized culture.
Research Area Metabolism, Microbiology, Bacteriology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Eiteman et al. 2009. Biotechnol Bioeng. 102(3):822-7. PMID: 18828178.

A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose.

Europe PMC ID: 18828178


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References: 2 entries

Eiteman et al. 2009. Biotechnol Bioeng. 102(3):822-7. PMID: 18828178.

A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose.


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