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Anti-Ferret immunoglobulin (IgI) [GK1-2-8-H9]

Info

Catalogue Number 153923
Applications ELISA FACS WB
Antigen/Gene or Protein Targets Ferret Immunoglobulin
Synonyms Ig
Relevance The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology. These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79β+ B cells. Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells, and represent great improvement over polyclonal anti-ferret Ig reagents.
Host Mouse
Immunogen Ferret IgG and IgM
Subclass IgG1 kappa
Notes These murine mAb specificity for ferret immunoglobulin (Ig). These mAb have a variety of documented research, and potentially veterinary, applications including flow cytometry (FCM), enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and Western blot (WB). These reagents address a currently unmet need because they enable discrimination of ferret B cells and derivative products (Ig) using mAb, whereas existing reagents are polyclonal in nature and finite in quantity. These mAb also enable segregation of ferret B cells by FCM on the basis of light chain expression, and thus are invaluable in diagnosis of B cell malignancies. Moreover, these mAb are amendable to intracellular staining and enable identification/enumeration of antibody-secreting cells (ASC) by FCM.
Research Area Immunology, Virology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Kirchenbaum et al. 2017. J Immunol. 199(11):3798-3807. PMID: 29079697.

Infection of Ferrets with Influenza Virus Elicits a Light Chain-Biased Antibody Response against Hemagglutinin.

Europe PMC ID: 29079697


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References: 2 entries

Kirchenbaum et al. 2017. J Immunol. 199(11):3798-3807. PMID: 29079697.

Infection of Ferrets with Influenza Virus Elicits a Light Chain-Biased Antibody Response against Hemagglutinin.


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