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Catalogue Number 153872
Backbone Size (bp) 11530
Bacterial Resistance Ampicillin
Selectable Markers Puromycin
Vector Type Mammalian Expression, Lentiviral, CRISPR
Antigen/Gene or Protein Targets Gt(ROSA)26Sor; ROSA26
Positive Control -
Relevance Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells.

gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785.

Species: Mus musculus (house mouse)
Cloning method: Restriction Enzyme
5’ cloning site: BsmbI
3’ cloning site: BsmbI
5’ sequencing primer: U6 Forward
Research Area Fluorescent Cell Imaging, Gene Expression

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